LAMP, NASBA, SDA, RCA and RPA are some of the isothermal amplification techniques which are preferable for POC diagnostics. Isothermal amplification techniques have been found suitable for POC techniques and follow WHO’s ASSURED criteria. The isothermal amplification methods are exploited to develop miniaturized sensors against viruses, bacteria, fungi and other pathogenic organisms and have been applied for in situ diagnosis. However, using bulky thermal cyclers and costly laboratory setup limits its uses in developing countries, including India. Efficient pathogen detection is a key to adequate food safety and hygiene.
PCR, a gold standard technique for amplifying nucleic acids, is widely used in scientific research and diagnosis. Nucleic acids are prominent biomarkers for diagnosing infectious pathogens using nucleic acid amplification techniques (NAATs).
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